Line-Shaped Illumination: A Promising Configuration for a Flexible Two-Photon Microscopy Setup

Year: 2022

Authors: Parravicini J., Hasani E., Tartara L.

Autors Affiliation: Dipartimento di Fisica & Astronomia, Universita di Firenze, IT-50019 Sesto Fiorentino, FI, Italy; LENS–European Laboratory for Nonlinear Spectroscopy, IT-50019 Sesto Fiorentino, FI, Italy; INO-CNR–Istituto Nazionale di Ottica del Consiglio Nazionale delle Ricerche, IT-50019 Sesto Fiorentino, FI, Italy; Dipartimento di Ingegneria Industriale e dell?Informazione, Universita di Pavia, IT-27100 Pavia, PV, Italy; Bright Solutions, IT-27100 Cura Carpignano, PV, Italy

Abstract: Featured Application Two-photon microscopy imaging, real-time two-photon imaging, nonlinear spectral measurements, dye characterization, biological sample imaging. An innovative two-photon microscope exploiting a line-shaped illumination has been recently devised and then implemented. Such configuration allows to carry out a real-time detection by means of standard CCD cameras and is able to maintain the same resolution as commonly used point-scanning devices, thus overcoming what is usually regarded as the main limitation of line-scanning microscopes. Here, we provide an overview of the applications in which this device has been tested and has proved to be a flexible and efficient tool, namely imaging of biological samples, in-depth sample reconstruction, two-photon spectra detection, and dye cross-section measurements. These results demonstrate that the considered setup is promising for future developments in many areas of research and applications.


Volume: 12 (8)      Pages from: 3938-1  to: 3938-11

More Information: This work was partially funded by CrestOptics.
KeyWords: two-photon microscopy; two-photon absorption; imaging technique; experimental technique; nonlinear spectra; dyes
DOI: 10.3390/app12083938